Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). . This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. . 99 to $1999. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. SpectraMax® i3x. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate. Download PDF. SpectraMax i3x, onboard luminescence 121. . The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 0´ 104 1. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Nucleic Acid and Protein Quantitation. Actual shake speed is based on the microplate format. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Also refer to this Multimode Reader Comparison Chart. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax i3x readers. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. . 305 reference level saturation. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. We would like to show you a description here but the site won’t allow us. Spectranet customer portal. Spectranet customer portal(SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. SpectraMax Microplate Readers. A: percent GFP-positive cells calculated using the classification feature in the software. pH-Xtra Glycolysis Assay - Agilent. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataSpectraMax i3x Multi-Mode Microplate Reader. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. Version 7. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Background noise is reduced by the reader’s cooled PMT, enabling increased sensitivity and dynamic range. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. As. MitoXpress-Xtra Oxygen Consumption Assay - Agilent. A 1:2 dilution series of BSA was prepared in PBS, starting at 100 mg/mL, in LoBind microcentrifuge tubes. Controls containing all reaction. 3: The cuvette chamber. ü; ü. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. SpectraMax ® iD3 and iD5 Multi-Mode . Sufficient for 1000 reactions. 吸収度マイクロプレートリーダー SpectraMax® Mini. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The calculated LOQ values were 0. Wokingham, Berkshire RG41 5TS United Kingdom. Tip: MCF-7 cells have a polygonal morphology with irregular dimensions, and they have a habit of clumping into large aggregates. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. SpectraMax i3x Multi-Mode Microplate Reader. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. 1 Microplate Data Acquisition & Analysis Software for Windows. Database contains 1 Molecular Devices SpectraMax i3x Manuals (available for free online viewing or downloading in PDF): Installation manual . The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 315 can't find zero order. 58% for the detection module and 2. 9 x 10-15. SpectraMax i3x Multi-Mode Detection Platform. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viabilitySoftMax® Pro 7. In this product video, see how the SpectraMax M5 quickly reads samples. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 0. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. Subsequently, 100 μL of the substrate solution (2 mM of p-nitrophenyl butyrate in dimethylformamide) was added into the mixture, and incubated at 37°C for 15 min. Figure 4. 3 STANDARDIZE TO A SINGLE ANALYSIS PLATFORMLearn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. 1321N1, a human astrocytoma cell line. The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Bioz Stars score: 98/100, based on 1 PubMed citations. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. 0001 0. . Figure 4. Instrument. Seller Details. 1. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. 7 Figures from 1126 Publications Promega. Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read). 9000-0756. HTRF IP standard curve. Molecular Devicesの全商品を見る. 1. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. 2. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). . TR-FRET ratios were calculated by dividing the RFU value for the 665-nm reading by the RFU value for the 616-nm reading, and the resulting numbers were multiplied by 1000. Contact Local Team. Order Now. Danaher Inc spectramax i3x. ZERO BIAS - scores, article reviews, protocol conditions and more. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. This process should take about 3 - 5 minutes. . Align the two slots on the bottle holder with the two pegs on the rail. The calculated LOQ values were 0. Future ready The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. 01 0. g. +44-118-944-8000. SpectraMaxParadigmMulti-ModeDetectionPlatformUserGuide 4 5014038F Chapter5:ReadModesandReadTypes 71 SupportedReadTypes 71 AbsorbanceReadMode 74 FluorescenceIntensityReadMode 79the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. . The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. If no sound is heard during initialization, contact technical support. StakMax Handling Stacker Base Unit. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideRecommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Learn more about its features, specifications, and applications in this user guide. Nuclear Receptor. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Features. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Whether you are looking for an intuitive, easy-to-use reader for. 0´ 104 4. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. No accessories are needed and SoftMax® Pro. For each kit, the reader was able to detect the lowest dilution as positive. Serial number 33 900 01-3387. detection cartridge LUM96: 59. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. ; SoftMax® Pro 7. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringFluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). SpectraDrop Abs Protein Quant (i3-i3x)Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. NORTH AMERICA. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. 1 1 10 100 e e e e e e eSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. Italic and boldface type are used for emphasis. Current methods available to rapidly detect these can be resource intensive and thus sub-optimal. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax i3x Multi-Mode Microplate Reader. 1. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. Description 1. It has a cooled PMT, a no-stain cell analysis algorithm, and a field-upgradeable imaging cytometer. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. 12911151) 0. i3x. The dynamic range of the assay is about 3. Download PDF. 과 같은 Parameter를 검사하여 평가할 수 있습니다. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. to View Figures. 1. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Settings not required by a reader are indicated by ‘---’. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SpectraMaxi3Multi. In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. 5mM EDTA, 1% NP40,Explore a wealth of applications with the future-ready SpectraMax i3x. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). compound concentration, and an IC 50 value of 21. Note: Additional readers with similar performance are listed. . Introduction. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Molecular Devices推出的SpectraMax® i3x 多功能酶标仪除了具有全波长的光吸收、荧光、化学发光和FRET检测功能以外，还兼容了客户端模块化升级功能，用户可以根据需要任何时间随意升级至荧光偏振FP、HTRF、AlphaScreen、Western Blot、上转发光检测、细胞成像和带有注射器. 6% for the on-board optics (monochromators). As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. Both readers feature an 8-channel read head for fast reads and. • Removing the transport locks from the PMT shutters and drawers. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. GloMax Discover System. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. . Prepare your iD series instrument for shipment. FTIR Spectrometer: Shimadzu IRSpirit (2018)The plate was read on the SpectraMax i3x Multi-Mode Microplate Reader. SpectraMax i3xは、吸光・蛍光・発光スペクトル測定のためのマルチモードマイクロプレートリーダーです。. Background Parallax,Columns 2. With optimized reagents, option to operate the. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. Filterless readers provide consistent results with steady temperature regulation. which chemiluminescence was detected on a SpectraMax i3x reader (Molecular Devices) and analyzed us ing GraphPad. Figure 1. The plate was shaken and incubated in the dark for 10 minutes. SpectraMax® ABS and ABS Plus reader support; ü. Table 1. Restart the system. 3) At the bottom of the strip, click on "Show. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Results for SpectraMax i3x reader with NanoBRET Detection Module (blue) and on-board luminescence detection mode (orange). . Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. モジュールを追加してアップグレードすることにより、ウェスタンブロットやイメージング、試薬インジェクタを用いた高速カイネティック測定を行う. SpectraMax i3x and Paradigm readers require the FP-FLUO. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. SoftMax Pro. 302 low ref light. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. 100 fmol 384-well (75 µL) 0. . Explore a wealth of applications with the future-ready SpectraMax i3x. 蛍光 発光 ELISA. Ease of use. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. 313 reference gain check fail. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. EUROPE. (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. The plate was then read on the SpectraMax iD5, SpectraMax i3x, and SpectraMax M5e readers using the settings in Table 1. Examples: “Press carefully to engage,” “ Do not. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Catalog excerpts. Price from $9. Average 98 stars, based on 1 article reviews. Products : SpectraMax i3x reader, SoftMax Pro GxP Software, SpectraMax i3, SoftMax Pro Software, SpectraMax MiniMax cytometer When I attempt a read with my SpectraMax i3 or i3x reader using the injector cartridge, why do I see a plate height error, even though I have entered the correct plate height?. Description 1. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. . The assay can accurately test the toxicology of compounds as shown in Figure 1. . Read height should be optimized for . Register to download your free eBook today. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. SPECTRAMAX i3X, INJECTOR MODULE CARTRIDGE model number 0200-7029. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. ZERO BIAS - scores, article reviews, protocol conditions and more. 314 low lamp level warning. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. Then, cells were washed with PBS three times and 100 μL of Nano-Glo HiBiT Lytic Reagent was added to. . Figure 4. 0´ 104 6. The solutions were thoroughly mixed and then incubated in the dark at 20°C for 2 h. . The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. 3. 303 unable to cal dark current. The treatments were. Supported Windows Operating System: See release notes. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. 2-µL or 4-µL samples were pipetted onto a 64-well SpectraDrop slide in quadruplicate, following the plate map in Figure 2. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. . 305 reference level saturation. Published Dec 16, 2020 | Updated Oct 10, 2023. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Multimode Reader Comparison Chart. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. Shake intensity. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. SeeSpectraMax MiniMaxImagingCytometeronpage85. Wait 20 seconds. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Monday to Friday 7am - 5pm Pacific Time. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Table 1. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Average 98 stars, based on 1 article reviews. User-upgradeable detection modules allow you to expand the capabilities of your microp. Promega. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. . Not compatible with Home Edition. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. 2 x 10-14. 2 only) SpectraMax M-series Microplate readers. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Eliminate time-dependent substrate addition steps. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Modularity does provide some measure of forward vision into upcoming or future experimental needs. Use the 3. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. The SpectraMax i3x reader offers a modular design allowing the addition of FP detection to the instrument in just minutes. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. 4. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. were measured at OD405 using a SpectraMax i3x multimode detection system (Molecular Devices, San Jose, CA, USA), and the melanin concentration was determined using the standard curve of melanin solutions with known concentrations. The information below applies to a standard 96 well plate, set to a linear shake mode. Ribonuclease (RNase) R digestion assay For identifying the stabilization of circVRK1, 2 μg isolated RNA from HBMECs was digested by RNase R (3 U/μg; RNR07250, Geneseed, Guangzhou,. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. 020 fmol 0. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Unplug the power cable from the back of the instrument. applications, including dual luciferase and ATP assays. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Find regional support. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. The Results. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. . using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. . The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. 304 signal level saturation. . . 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideDiscover how the automation-friendly SpectraMax® i3x Multi-Mode Microplate Reader, designed to expand research capabilities through its versatile and user-upgradeable application modules, can. 7. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. china@moldev. More Product Information. Failed to load PDF file. 312 gain calibration failed. Image analysis provides accurate data. Figure 1. ü; ü. . )wideand65. . This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Unparalleled performance on a personalized platform. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. com. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Determination of anti-inflammatory. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Tristar 5 Multimode Microplate Reader. Eliminate time-dependent substrate addition steps. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application. infoboxeu@moldev. 99. STAKMAX. to View Figures. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The StemoniX microBrain 3D platform is a homogenous 3D neural spheroid system in 96 and 384-well format. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. SpectraMax Quant dsDNA Assay Kits. 303 unable to cal dark current. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3.